1. Basic concepts of primer designing: a mini review
Authors : Mona Sharma
Pages : 10-12
DOI : http://dx.doi.org/10.21172/1.174.03
Keywords : PRIMERS, BLAST, PCR Abstract :Primer design is an important pre-requisite for many of the molecular biology experiments. It certainly requires using multiple databases and bioinformatics tools to identify sequence of interest. The essential features of ‘good’ primers need to be identified first before proceeding further for any molecular technique. Amplification of the target of interest is based on the fact that the primer matches specifically to the target and there is no non-specific amplification. The process of primer designing is not an easy process and essentially completes in two parts. Initially primers flanking the regions of interest are generated and then using tools such as BLAST these regions are searched to analyse the potential targets. The analysis is complex and time-consuming as number of technical observations is to be made and the problem becomes harder when large numbers of the primer hits are observed. Therefore, understanding the principles of primer designing are essential to tackle the possible troubleshoots. This review highlights the basic fundamentals of primer designing.
Citing this Journal Article :Mona Sharma, "Basic concepts of primer designing: a mini review", Volume 17 Issue 4 - October 2020, 10-12
Click here to Submit Copyright Takedown Notice for this article.