Volume 13 Issue 3 - May 2019

  • 1. Description of cochliopodium massiliensis sp. nov., isolated in bronchoalveolar lavage of a tunisian patient with pneumonia.

    Authors : Hanene Saadi, Gabrielle Boussay, Maryse Loinard, Akila Boussaid, Mondher Boughalmi

    Pages : 10-15

    DOI : http://dx.doi.org/10.21172/1.133.02

    Keywords : Cochliopodiidae, Cochliopoduim, ultrastructure, comparative morphology, phylogeny, SSU-rDNA, taxonomy.

    Abstract :

    In this work, we describe the isolation of Cochliopodium massiliensis sp nov in a bronchoalveolar lavage from a patient with pneumonia. It is the first international isolate of this genus of amoeba in a human sample but also the first case described during this pathology. This new species is the smallest of the Cochliopodium species already described. Cochliopodium massiliensis sp nov is described on the basis of morphological observations in light microscopy, ultrastructure by TEM microscopy and molecular genetic evidence of SSU rDNA. Cochliopodium massiliensis n. sp (length ~ 16μm) is characterized by the presence on the surface of complex structures called Scale. Containing a apical funnel shaped collar (~560 nm), dorsal and ventral bases plate of the Scale (with radius ~ 420 nm). Based on morphological and molecular genetic data of SSU-DNA gene, we describe our isolate as a new species, the first isolate of this genus from a human biological sample, and the first case described during pneumonia.

    Citing this Journal Article :

    Hanene Saadi, Gabrielle Boussay, Maryse Loinard, Akila Boussaid, Mondher Boughalmi, "Description of cochliopodium massiliensis sp. nov., isolated in bronchoalveolar lavage of a tunisian patient with pneumonia.", Volume 13 Issue 3 - May 2019, 10-15